The previous lock had just expired, which I had anticipated hence me traveling back to it to re-lock it.  So, I went to re-lock it, and so mere seconds after I did, my lock was somehow removed by the approaching enemy.  Even if it hadn't expired yet though as I went to apply another lock, one can re-lock an already locked flag.

Not in my experience. At the very least, it doesn't seem to reset/add to the duration. I locked an already locked flag as it was about to expire, and it did expire a few seconds later.

It seemed to be the Regulus that was able to do it... (I'm one of the team mates)

I don't think it was throwing mines at the already locked flag that did it. In fact, I'm not sure... I even bought some restorative scrolls (the debuff remover) and that didn't do it... I'm fairly certain it was an exploit....

I am %100 certain that the 45 seconds didn't expire before the flag lock was gone... Weird ish, definately cost us the game though. (When you have Giants on the way and you have their flags locked it should be GG, until that happens, and they remove our locks on our ports!)

Are you sure, seems to me like they do stack, maybe not for 100%. But what i saw wasnt a problem with stacking locks. We didnt keep dropping them.

In any case, that wasn't the issue.  The issue was that once one of our flags expired, we re-locked it only to find the lock somehow removed after 3 seconds instead of 45.  My only real concern is that some people out there in the community may know of an exploit to remove freshly applied flag locks.

People are getting off-topic here.

The OP said the previous lock had expired, so he moved to the flag, and slapped another one onto it. This was then somehow removed.

Ps, are you sure the flag wasn't going past the neutral bar at the presice momment you locked it? Cos i've seen that happen before and the flag lock becomes void.

From looking trough the LUA code the only thing I can see would be that the graphics are turned off after the first 45 secs, the real lock should still be active though.

                        # Start Can Not Capture Flag Effect
                        AttachEffectsAtBone( params.Targets[1], EffectTemplates.Items.Consumable.DefenseOfVayikraActivate, -2 )
                        WaitSeconds(45)

If anyone could care to test this by casting a flaglock on an already existing one about 30 secs old and see if the graphics are removed 15 secs later. The flag should still be uncapturable though since the capture buff stacks Always

             Buffs = {
                BuffBlueprint {
                    Name = 'Item_Consumable_030',
                    DisplayName = '<LOC ITEM_Consumable_0016>Capture Lock',
                    Description = '<LOC ITEM_Consumable_0032>Cannot be captured.',
                    BuffType = 'IUSESPRINT',
                    Debuff = false,
                    Stacks = 'ALWAYS',
                    Duration = 45,
                    Affects = {
                    },
                    Icon = 'NewIcons/Gadget/DefenseofVayikra',

Did you read the thread? It happens after the old lock has dissapeared when you try to put a new lock on.

As in there is no lock shown on the flag, and you use a capture lock on the flag. It then dissapears after a few seconds.

If by "expired" you mean that the cooldown was over in your consumable belt, that doesn't matter. You have to wait for the lock to physically disappear from the flag and cap it again. If the lock animation is still on the flag, you will indeed lose your lock that you just applied, effectively wasting it.

Aside from that, I can't think of anything else other than an exploit that could do something like that.

in short - no there is not a way to remove flag locks, so it is most likely you applied it too quickly and due to lag or whatever it got removed by the timer from the previous lock, next time wait a few seconds after the lock dissappears from the flag before applying the next flag lock

It'd be nice if I could use a flag lock to unlock the flag that was previously locked by the opposing team.

You should have to buy Keys.  They should cost 500.